rna preparation and microarray analysis (Welgene inc)
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Rna Preparation And Microarray Analysis, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Identification of novel genetic regulations associated with airway epithelial homeostasis using next-generation sequencing data and bioinformatics approaches"
Article Title: Identification of novel genetic regulations associated with airway epithelial homeostasis using next-generation sequencing data and bioinformatics approaches
Journal: Oncotarget
doi: 10.18632/oncotarget.19752
Figure Legend Snippet: Gene expressions of the (A) upregulated and (B) downregulated genes shown in Table were analyzed using GSE43696 microarray data from the GEO database. The results showed that gene expressions of both MEF2C and MDGA1 were significantly downregulated in patients with either mild-moderate or severe asthma compared to normal controls. n.s. means no significance, * represents p-value < 0.05, ** represents p-value < 0.01, and *** represents p-value < 0.001. (Probe information of GSE43696 array from GEO database: FGF2-#1, A_23_P218918; FGF2-#2, A_24_P931472; TP53I11, A_23_P368028; MDGA1, A_23_P310460; MEF2C, A_23_P320739; IRAK3, A_23_P162300; LOX, A_23_P122216; NEURL1-#1, A_23_P138492; TFPI-#1, A_23_P156826; TFPI-#2, A_23_P330070; TFPI-#3, A_23_P17095; NAIP, A_23_P110473.)
Techniques Used: Microarray
Figure Legend Snippet: Gene expressions of the 13 upregulated genes shown in Table were analyzed using GSE43696 microarray data from the GEO database. None of these 13 genes were significantly upregulated in patients with asthma. n.s. means no significance, * represents p-value < 0.05, ** represents p-value < 0.01, and *** represents p-value < 0.001. (Probe information of GSE43696 array from GEO database: OCLN, A_23_P92672; CLDN1, A_23_P57784; NLGN4X-#1, A_23_P364592; XYLT1, A_24_P787897; FGFR3, A_23_P500501; COL5A1-#1, A_23_P158593; COL5A1-#2, A_23_P83818; MMP9, A_23_P40174; SCIN, A_23_P157136; CACNA1A-#1, A_24_P130559; RGS2, A_23_P114947; LMO2, A_23_P53126; UTY, A_23_P329835; EPB41L3, A_23_P4536.)
Techniques Used: Microarray
Figure Legend Snippet: Gene expressions of the 40 downregulated genes shown in Table were analyzed using GSE43696 microarray data from the GEO database. The results showed that KCNJ2 expression was significantly downregulated in patients with severe asthma. n.s. means no significance, * represents p-value < 0.05, ** represents p-value < 0.01, and *** represents p-value < 0.001. (Probe information of GSE43696 array from GEO database: KCNJ2, A_23_P329261; FN1, A_24_P334130; FOS, A_23_P106194; PTGER1, A_23_P4808; RAC2, A_ 23_P218770 ; FZD2, A_23_P141362; GLI2, A_23_P209246; RAC3-#1, A_23_P125001; RASGRP2-#1, A_23_P64058; EGLN3-#1, A_23_P360379; FGF13, A_23_P217319; HHIP, A_23_P167129; MMP1, A_23_P1691; CHRM4-#1, A_23_P104845; MYLK, A_23_P143817; HLA-DQB1, A_23_P8108; NTNG1-#1, A_23_P201547; NTNG1-#12, A_24_P359671; CNTN1, A_23_P390700; ITGB2, A_23_P329573; ITGA4, A_23_P56505; HLA-F-#1, A_23_P145264; HLA-F-#2, A_23_P145264; HS3ST3A1, A_23_P66525; HS3ST2, A_23_P118158; HS3ST3B1-#1, A_23_P77918; LAMA1, A_32_P313405; COL4A2, A_23_P205031; COL1A2, A_24_P277934; COL6A1, A_32_P32254; DUSP10, A_24_P182494; KCNMB4, A_23_P64792; ATP1A4, A_23_P160177; ATP1A1, A_23_P1072; ADRA2C, A_23_P256158; FLI1, A_24_P355649; NGFR, A_23_P389897; MMP3, A_23_P161698; TLR4, A_24_P69538; KCNJ12, A_24_P339429; ITGA4, A_23_P56505; HTR1F-#1, A_23_P166674; PTPRB, A_23_P53390.)
Techniques Used: Microarray, Expressing
Figure Legend Snippet: Functional analysis of 504 differentially expressed genes discovered from the GE microarray data was performed by functional annotation (Biological Processes) in DAVID database. The results showed that these genes were involved in cell adhesion (26 genes), keratinization (9 genes), keratinocyte differentiation (11 genes), proteolysis (30 genes), collagen catabolic process (12 genes), extracellular matrix organization (20 genes), and peptide cross-linking (12 genes). The selected criteria were EASE = 0.1, p-value < 0.05, and fold enrichment > 1.3.
Techniques Used: Functional Assay, Microarray